Equipment

Iris Pharma’s laboratories are equipped with state-of-the-art technology to provide our customers with high-quality, reliable and accurate data in the shortest possible time.

We use cutting edge equipment, including optical coherence tomography (OCT), to perform experiments in our own GLP-compliant facilities.

Our preclinical laboratory staff is trained for micro-dissection of ocular samples, as well as surgical administration and implantation of ophthalmic drugs and devices. 

Preclinical research

We use state-of-the-art ocular technology to demonstrate the efficacy of particular ophthalmic drugs or device:

  • Retinal function: Electroretinograph (ERG), Spectralis®HRA+OCT.
  • Surgery: operating microscope, video digital camera slit-lamp, numeric slit lamp, argon laser.
  • Imaging: Apotome, Cyclone Plus Phosphor Imager.
  • Ocular inflammation and tolerance measurement: Microsphere-based liquid array (Luminex Lx200), laser flare meter, Heidelberg Retina Tomograph II (HRT II) with corneal, retina and glaucoma modules.
  • Intra-Ocular Pressure (IOP) measurement: Pneumatonometer, TonoLab, Tonopen.
  • Choroidal angiogenesis: Heidelberg Retina Angiography I (HRA 1).
  • Vascular permeability and tolerance measurement: Fluorotron Master.

OCT retina

Bioanalysis

ELISA

We are experts in processing samples using tools that are both fast and sensitive, such as:

  • Triple-quadrupole mass spectometer (RRLC-MS/MS)
  • Microsphere-based liquid array (Luminex Lx200)
  • High-performance liquid chromatography (HPLC) coupled with different detectors (MS, RID, FLUO, UV)
  • Radioactivity counter (beta and gamma)
  • Biochemistry analyzer: Piccolo Xpress
  • Hematology analyzer: MS9-5 
  • Plate reader for ELISA 

Histology

All materials for histology and bacteriology are available in our facilities (e.g. an ApoTome fluorescence microscope, a microtome, a cryocut and a fully Automated Staining System).

Ocular histology

Radioactive assay

Iris Pharma is authorized to handle and use radiolabeled compounds.

  • Radioactivity counters (beta, gamma).
  • Cyclone Plus Phosphor Imager.
  • Storage phosphor system.

Latest acquisitions

  • Spectralis®HRA+OCT: very high precision monitoring of the areas of the retina which have been treated and their evolution.
  • Microsphere-based liquid array (Luminex Lx200): biochemical quantifications (e.g. cytokines in the in vivo model of endotoxin-induced uveitis) and multiple simultaneous analyses in low sample volumes.
  • Counter beta Tri-Carb 2900: liquid scintillation analyzer. This instrument measures levels of radioactive elements by counting photonic emissions produced when alpha and beta particles interact with certain reagents (scintillants).
  • Cyclone Plus Phosphor Imager: radiometric phosphor imager for quantitative filmless autoradiography.
  • ApoTome: light microscope using "structured illumination" process for 3D fluorescence imaging.
  • RRLC-MS/MS: triple quadrupole mass spectrometer. Mass spectrometry is used for the structural characterization and pharmaceutical profiling of all new compounds. Another important application area is the identification of drug metabolites. RRLC-MS/MS system dramatically increases the speed of analysis.
  • Oxycycler: a computer-controlled chamber used for the Retinopathy Of Prematurity (ROP) animal model. Litters are placed into an incubator attached to the Oxycycler who regulates the atmosphere inside by injecting oxygen.